Flanker Chris Robshaw and full-back Mike Brown are questions for England’s Six Nations opener against Italy.
Robshaw, 31, has back damage, while his Harlequins partner Brown, 32, is hampered by obscured vision, taking England’s damage rundown to 15 players.
Head mentor Eddie Jones said the combine were both “fringe” to partake in the competition opener on 4 February.
On Tuesday, Harlequins tight-head Kyle Sinckler pulled back through damage and was supplanted by Bath’s Henry Thomas.
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“Robshaw has invested a great deal of energy in the pool, however, he’s fringe to play rugby. Dark colored is doing light abilities yet he’s marginal as well,” said Jones on Wednesday.
Britain has won every one of the 23 gatherings with Italy and will be solid top choices to keep up their 100% record when they dispatch their offer for a third Six Nations title in succession.
Darker, who has played 64 times for his nation, scored an attempt in England’s last trip, November’s 48-14 prevail upon Samoa at Twickenham.
Previous chief Robshaw likewise played the whole 80 minutes in that diversion, which was his 59th top.